cell-free-expression
Provides guidance for optimizing cell-free protein synthesis experiments, addressing yield issues and DNA template design.
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The cell-free-expression skill was audited on May 31, 2026 and we found 19 security issues across 1 threat category. Review the findings below before installing.
Categories Tested
Security Issues
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External URL reference
SourceSKILL.md
| 254 | - [User's Guide to CFPS - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC6481089/) |
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SourceSKILL.md
| 255 | - [Optimising Protein Synthesis in Cell-Free Systems - PMC](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996726/) |
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SourceSKILL.md
| 256 | - [CFPS Systems Comparison - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC8258279/) |
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SourceSKILL.md
| 259 | - [Crude Extract Preparation - MDPI Methods](https://www.mdpi.com/2409-9279/2/3/68) |
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SourceSKILL.md
| 260 | - [Simple Rapid Cell-Free Lysate - PLOS One](https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165137) |
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SourceSKILL.md
| 261 | - [High-Throughput Extract Preparation - Nature Scientific Reports](https://www.nature.com/articles/srep08663) |
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SourceSKILL.md
| 264 | - [PURE System Evolution - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC10521753/) |
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SourceSKILL.md
| 265 | - [PURE System for Membrane Proteins - Nature Protocols](https://www.nature.com/articles/nprot.2015.082) |
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SourceSKILL.md
| 268 | - [Wheat Germ Systems Review - FEBS Letters](https://febs.onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.05.061) |
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SourceSKILL.md
| 269 | - [Wheat Germ for Structural Biology - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC8027086/) |
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SourceSKILL.md
| 272 | - [Rare Codons and Solubility - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC2723077/) |
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SourceSKILL.md
| 273 | - [Codon Influence on Expression - Nature](https://www.nature.com/articles/nature16509) |
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SourceSKILL.md
| 274 | - [Synonymous Codon Substitutions Perturb Folding - PNAS](https://www.pnas.org/doi/10.1073/pnas.1907126117) |
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SourceSKILL.md
| 277 | - [Oxidative Protein Folding in ER - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC4312752/) |
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SourceSKILL.md
| 278 | - [PDI-Regulated Disulfide Formation - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC7794689/) |
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SourceSKILL.md
| 281 | - [SUMO Fusion for Difficult Proteins - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC7129290/) |
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SourceSKILL.md
| 282 | - [Fusion Tags Review - Frontiers Microbiol](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00063/full) |
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SourceSKILL.md
| 285 | - [Cold Shock Promoters - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC9800685/) |
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SourceSKILL.md
| 286 | - [Strategies to Optimize E. coli Expression - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC7162232/) |
Scanned on May 31, 2026
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Categoryeducation research
UpdatedJune 15, 2026
FridrichMethod/awesome-skills