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binding-characterization

Provides guidance for SPR and BLI binding characterization experiments, aiding in planning, troubleshooting, and data interpretation.

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The binding-characterization skill was audited on Feb 12, 2026 and we found 11 security issues across 1 threat category. Review the findings below before installing.

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Security Issues

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External URL reference

SourceSKILL.md
216- [BLI vs SPR Comparison - Sartorius](https://www.sartorius.hr/en/news/blog/bli-vs-spr-choosing-the-ideal-method-for-analyzing-biomolecular-interactions/)
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SourceSKILL.md
217- [BLI vs SPR - Nicoya](https://nicoyalife.com/blog/biolayer-interferometry-vs-surface-plasmon-resonance/)
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SourceSKILL.md
220- [SPR Guidelines - van der Merwe, Oxford](https://www.path.ox.ac.uk/wp-content/uploads/2023/09/SPR-guidelines-1.pdf)
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SourceSKILL.md
221- [SPR Experiment Guide - Duke DHVI](https://dhvi.duke.edu/sites/default/files/2022-08/SPR%20Experiment%20Guide%20v1.3.pdf)
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SourceSKILL.md
224- [4 Ways to Reduce NSB in SPR - Nicoya](https://nicoyalife.com/blog/4-ways-reduce-non-specific-binding-spr/)
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SourceSKILL.md
225- [3 Ways to Limit Mass Transfer Effects - Nicoya](https://nicoyalife.com/blog/3-ways-to-limit-mass-transfer-effects/)
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SourceSKILL.md
226- [Suppressing NSB in BLI - ACS Omega](https://pubs.acs.org/doi/10.1021/acsomega.1c05659)
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SourceSKILL.md
229- [SPR Regeneration - SPRpages](https://www.sprpages.nl/kinetics/regeneration)
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SourceSKILL.md
230- [Mastering Regeneration - Nicoya](https://nicoyalife.com/blog/regeneration-buffer-spr-experiment/)
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SourceSKILL.md
233- [Mass Transport Limitation in SPR - PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC4134667/)
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SourceSKILL.md
234- [Mass-Transfer Kinetics - SPRpages](https://www.sprpages.nl/data-fitting/kinetic-models/mass-transfer)
Scanned on Feb 12, 2026
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Categoryeducation research
UpdatedMay 21, 2026
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